1. Firstly, we must know whether the filter material has influence on the filter membrane. 2. According the material choose the suitable filter membrane. 3. According to your filter material and process requirements choose the suitable membrane pore size. 4. Usually, there is no positive or negative side for membrane, but it's better to filter the material from the smooth side to the rough side. 5. Filtered solvents should be keep properly to avoid secondary pollution. 6. The waste membrane should be collected together and then process to avoid environmental pollution.

Nylon syringe filter is ideal for aqueous(non-acid) or organic sample prep and HPLC or dissolution sample analysis. MCE syringe filter suitable for aqueous protein solutions. PES syringe suitable for pre-filtration and filtration of buffers and culture media. Hydrophobic PTFE syringe filter usually used in organic solvent with strong chemical causticity filtration. Hydrophilic PTFE syringe filter is versatile filter for aqueous and aggressive organic solvent-based solutions and especially ideal for HPLC operation. PVDF syringe filter for filtration of non-aggressive aqueous and mild organic solutions, or were maximizing protein recovery is important.